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. 2020 Jun 3;10:9024. doi: 10.1038/s41598-020-65920-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-490-3p inhibited NSCLC progression via MAPK1. (A) Bioinformatics tools helped to find that miR-490-3p and MAPK1 3ʹ-UTR saw the existence of complementary binding sites. (B,C) Luciferase reporter assay confirmed that miR-490-3p and MAPK1 3ʹ-UTR exhibited molecular binding in A549 cells (B) and H1299 cells (C). (D,E) qRT-PCR assay found the existence of mRNA MAPKP level in indicated NSCLC cells. *P < 0.05; **P < 0.01; ***P < 0.001.