Figure 4: 5,15-diHpETE, but not LXB4, inhibits collagen-mediated platelet aggregation.
Isolated human platelets were treated with increasing concentrations of either LXB4 or 5,15-diHpETE for ten minutes and then stimulated with collagen (0.25 µg/mL). A) Representative tracings of 5,15-diHpETE or LXB4 treated platelets stimulated with collagen. B) Data represents mean ± S.E.M. Statistical analysis was performed comparing oxylipin concentration to vehicle control using one-way ANOVA with Dunnett’s multiple comparison test. *P<0.05, **P<0.01, ***P<0.001.