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Steady state kinetics and product profile of wt-hALOX12 and its mutants with arachidonic acid to test the aromatic interactions between these residues and the substrate.
| Enzymes | KM (µM) | kcat (s−1) | kcat/KM (µM)−1s−1 | Products (%) | |
|---|---|---|---|---|---|
| 12-HETE | 15-HETE | ||||
| wt-hALOX12 | 1.9 ± 0.2 | 28 ± 0.9 | 14 ± 0.9 | 100 ± 5 | 0 |
| F414L | 1.4 ± 0.2 | 1.7 ± 0.06 | 1.2 ± 0.1 | 97 ± 4 | 3 ±1 |
| F414W | 4.0 ± 0.3 | 16 ± 0.6 | 4.0 ± 0.2 | 100 ± 5 | 0 |
