. Author manuscript; available in PMC: 2020 Jun 4.

Published in final edited form as: Biochemistry. 2019 Jan 4;58(6):848–857. doi: 10.1021/acs.biochem.8b01167

Table 6.

Steady - state kinetics parameters of wt-hALOX12 and the mutants that makes the binding cavity wider (L407A, L407G) and wider as well as smaller (L407G/A417I/V418M).

Protein	K_M (uM) (AA)	k_cat (s⁻¹) (AA)	k_cat/K_M (uM)⁻¹s⁻¹ (AA)	Products with AA (%)
Protein	K_M (uM) (AA)	k_cat (s⁻¹) (AA)	k_cat/K_M (uM)⁻¹s⁻¹ (AA)	12HpETE	11HpETE	15HpETE
Wt-hALOX12	1.9 ± 0.2	28 ± 0.9	14 ± 0.9	100 ± 5	0	0
L407A	2.4 ± 0.2	0.28 ± 0.01	0.12 ± 0.01	91 ± 4	6 ± 1	3 ± 1
L407G	1.8 ± 0.3	0.22 ± 0.01	0.12 ± 0.02	60 ± 3	19 ± 2	21 ± 2
L407G/A417I/V418M	1.5 ± 0.3	0.19 ± 0.01	0.13 ± 0.02	66 ± 3	3 ± 1	31 ± 2