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. 2020 Jun 3;10:9054. doi: 10.1038/s41598-020-65680-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T47D cells are the most sensitive to radiation-induced cell death. (a–c) MTT assay evaluating cell viability of MDA-MB-231 (a), SKBR3 (b) and T47D (c) exposed to different radiation doses (2, 6, and 10 Gy) and evaluated 24, 48, or 72 hours after the irradiaition. The LD50 reported in the Fig (a-c). was caculated for each cell line four days after irradiation. Data represent the mean ± standard deviation (SD) of three experiments, each performed in triplcate. Statistical significance was evaluated using two-way analysis of variance (ANOVA), with Tukey’s multiple comparison test. (d) Survival curve of MDA-MB-231 and T47D cell evaluated by their colony forming ability using the indicated radiation doses. Data represent the mean ± SD of two independent experiments, each performed in triplicate. Multiple t-test, using the Holm-Sidak method, was used to statistical comparison. *p < 0.05, **p < 0.01, ***p < 0.001 and ns (not significant).