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. 2020 Jun 3;10:9054. doi: 10.1038/s41598-020-65680-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Signal transduction pathways are differently activated by radiation in T47D and MDA-MB-231 cells. (a) Western blot analysis evaluating the expression of the indicated phospho-proteins in T47D and MDA-MB-231 irradiated with 0.5 and 2 Gy doses and the harvested at the indicated time points. Vinculin was used as loading control. (b) Western blot analysis evaluating the expression of γH2AX and RAD51 in cells irradiated with 2 Gy and then collected at the indicated time points. Full-length blot is shown in Supplementary Fig. S1. Vinculin and GAPDH were used as loading control. NIR = Non-Irradiated cells.