TABLE 2.
Microbiological characteristics of nine clinical CPE isolates and their transconjugants.
| Isolate | Species | ST | MIC (mg/L) | Antimicrobial resistance phenotype | β-lactamase(s) | Size of plasmids | Inc group | ||
| ERT | MEM | IPM | |||||||
| LO-35 | E. coli | 410 | 0.5 | 0/125 | >4 | CAZ, CTX, FEP, CIP | OXA-48, CTX-M-15 | ∼39 kb | IncL/M |
| Tc-LO-35a | – | 0/125 | 0/125 | >4 | CAZ, CTX, FEP | OXA-48, CTX-M-15 | ∼39 kb | IncL/M | |
| LO-89 | K. pneumoniae | ST147 | 8 | 16 | 32 | CAZ, CTX, FEP, AMK, GEN, CIP | NDM-1, CTX-M-15, TEM, SHV | 104.8 ± 3.6 | IncFII |
| Tc-LO-89a | – | 4 | 8 | ND | CAZ, CTX, FEP | NDM-1, CTX-M-15, TEM | – | IncFII | |
| LO-112 | S. marcescens | – | 8 | 16 | 4 | CAZ, CTX, FEP, CIP | OXA-48, CTX-M-15, TEM, SHV | ∼39 kb | IncL/M |
| Tc-LO-112a | – | 4 | 4 | 2 | CAZ, CTX, FEP | OXA-48, CTX-M-15, TEM | ∼39 kb | IncL/M | |
| LO-149 | K. pneumoniae | ST15 | 8 | 32 | 32 | CAZ, CTX, FEP, AMK, GEN, CIP | NDM-1, OXA-48, CTX-M-15, TEM, SHV | 130.6 ± 3.2 | IncFII, IncL/M |
| Tc-LO-149a | – | 8 | 16 | 8 | CAZ, CTX, FEP, AMK, GEN | NDM-1, SHV | 130.6 ± 3.2 | IncFII | |
| LO-155 | K. pneumoniae | ST893 | 8 | 32 | 32 | CAZ, CTX, FEP, CIP | NDM-1, OXA-48, CTX-M-15, TEM, SHV | – | IncL/M |
| Tc-LO-155a | – | 8 | 8 | >4 | CAZ, CTX, FEP | OXA-48, CTX-M-15, TEM | – | IncL/M | |
| LO-179 | K. pneumoniae | ST16 | 8 | 32 | 24 | CAZ, CTX, FEP, AMK, GEN, CIP | NDM-1, CTX-M-15, TEM, SHV | – | IncFII |
| Tc-LO-179a | – | 4 | 4 | ND | CAZ, CTX, FEP, AMK, GEN | NDM-1, TEM | – | IncFII | |
| LO-204 | K. pneumoniae | ST15 | 8 | 32 | 256 | CAZ, CTX, FEP, AMK, GEN, CIP | NDM-1, OXA-48, CTX-M-15, TEM, SHV | 140.2 ± 3.2 135.1 ± 3.0 | IncFII, IncL/M |
| Tc-LO-204a | – | 8 | ND | ND | CAZ, CTX, FEP, AMK, GEN | NDM-1, OXA-48, TEM, SHV | 140.2 ± 3.2 135.1 ± 3.0 | IncFII, IncL/M | |
| LO-271 | K. pneumoniae | ST147 | 8 | 16 | 32 | CAZ, CTX, FEP, AMK, GEN, CIP | NDM-1, CTX-M-15, TEM, SHV | 107.4 ± 4.6 86.3 ± 4.8 | IncFII |
| Tc-LO-271a | – | 4 | 8 | ND | CAZ, CTX, FEP | NDM-1, CTX-M-15, TEM | – | IncFII | |
| LO-273 | E. cloacae | ST78 | 8 | 32 | 24 | CAZ, CTX, FEP, AMK, GEN, CIP | NDM-1, CTX-M-15 | – | IncFII |
| Tc-LO-273a | – | 8 | ND | ND | CAZ, CTX, FEP, AMK, GEN | NDM-1 | ∼50 kb | IncFII | |
MIC, minimal inhibitory concentrations; ERT, ertapenem, MEM, meropenem; IPM, imipenem; CAZ, ceftazidime; CTX, cefotaxime; FEP, cefepime; AMK, amikacin; GEN, gentamicin, CIP, ciprofloxacin, CST, colistin. aTc, E. coli K12 transconjugants selected in media containing 1 μg/ml MEM. Plasmid size for LO-89, LO-149, LO-204, and LO-271 isolates was estimated by ODM; that for other isolates was estimated by plasmid preparation.