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. 2020 Jun 3;10:9060. doi: 10.1038/s41598-020-65986-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histochemical analysis of the testis from each of the eight KO founders. PNA-LHC (green), γH2AX (red), and DAPI (blue) staining of the testis section of Ctag2 (a), 4930447F04Rik (b), Mir743 (c), Mir465 cluster (d), Gm1140 (e), Gm14692 (f), 4933436I01Rik (g), and Gm6812 (h) KO founders. All show sperm in lumen of seminiferous tubule and sex body formation. Scale bar: 50 µm.