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. 2020 Jun 3;10:9060. doi: 10.1038/s41598-020-65986-y

Table 2.

Generation of each the HS candidate gene KO founders by CRISPR/Cas9 system.

Gene Number of embryos Number of pups Number of founders
Injected Transferreda Newbornb Weaningc Birthrateb/a Male Female Totald Mutation efficiencyd/c
Ctag2 83 80 31 31 38.8% 13 8 21 67.7%
4930447F04Rik 84 82 27 27 32.9% 13 7 20 74.1%
Mir743 155 146 41 38 28.1% 7 7 14 36.8%
Mir465 cluster 75 72 26 24 36.1% 4 3 7 29.2%
Gm1140 266 249 38 36 15.3% 2 1 3 8.33%
Gm14692 100 98 18 16 18.4% 1 2 3 18.8%
4933436I01Rik 105 90 32 31 35.6% 10 7 17 54.8%
Gm6812 112 90 6 6 6.67% 1 2 3 50.0%