Table 4.
Efficiency of Agrobacterium infection of calli induced from immature embryos of 16 wild Oryza species
| Strain Centrifugation | LBA4404 | EHA105 | |||
|---|---|---|---|---|---|
| 1100×g | 20,000×g | 1100×g | 20,000×g | ||
| 1 | O. barthii, W1467, AA | 1/9 | 2/8 | 0/8 | 1/8 |
| 2 | O. glumaepatula, W2184, AA | 3/8 | 3/9 | 1/8 | 2/8 |
| 3 | O. longistaminata, W1573, AA | 0/8 | 0/8 | 0/8 | 1/8 |
| 4 | O. meridionalis, W2080, AA | 0/9 | 1/8 | 0/8 | 0/8 |
| 5 | O. rufipogon, W1551, AA | 3/8 | 2/8 | 0/8 | 1/8 |
| 6 | O. punctata 2x, W1582, BB | 2/8 | 0/8 | 0/8 | 0/8 |
| 7 | O. eichingeri, W1525, CC | 0/8 | 0/8 | 1/8 | 0/8 |
| 8 | O. officinalis, W1301, CC | 0/16 | 0/16 | 0/17 | 0/16 |
| 9 | O. rhizomatis, W1808, CC | 0/16 | 0/15 | 0/16 | 0/17 |
| 10 | O. minuta, W0051, BBCC | 0/8 | 0/8 | 1/8 | 0/8 |
| 11 | O. punctata 4x, W1564, BBCC | 0/8 | 0/8 | 1/8 | 0/8 |
| 12 | O. alta, W0017, CCDD | 1/8 | 1/8 | 0/8 | 0/8 |
| 13 | O. grandiglumis, W2220, CCDD | 0/16 | 0/18 | 0/16 | 0/18 |
| 14 | O. latifolia, W1177, CCDD | 0/24 | 0/25 | 0/24 | 0/24 |
| 15 | O. brachyantha, W1711, FF | 2/9 | 1/8 | 0/8 | 1/8 |
| 16 | O. longiglumis, W1227, HHJJ | 0/24 | 0/24 | 0/25 | 0/24 |
| 17 | O. sativa, Nipponbare, AA | 88/90 | 76/79 | 77/83 | 81/85 |
number of GFP positive calli / total number of tested immature embryos