Table 1.
16S rRNA gene-targeted specific primers used for real-time PCR
| Target Bacteria | Oligonucleotide sequence | Amplicon size (bp) | Reference |
|---|---|---|---|
| Escherichia | F: CATTGACGTTACCCGCAGAAGAAGC | 190 | Bartosch (2004) |
| R: CTCTACGAGACTCAAGCTTGC | |||
| Prevotella | F: CACCAAGGCGACGATCA | 283 | Larsen (2010) |
| R: GGATAACGCCYGGACCT | |||
| Lactobacillus | F: AGCAGTAGGGAATCTTCCA | 341 | Kanno (2009) |
| R: CACCGCTACACATGGAG | |||
| Bifidobacterium | F: TCGCGTCYGGTGTGAAAG | 243 | Rinttila (2004) |
| R: CCACATCCAGCRTCCAC | |||
| Akkermansia | F: CAGCACGTGAAGGTGGGGAC | 329 | Schneeberger (2015) |
| R: CCTTGCGGTTGGCTTCAGAT | |||
| Roseburia | F: TACTGCATTGGAAACTGTCG | 230 | Larsen (2010) |
| R: CGGCACCGAAGAGCAAT | |||
| Fecalibacterium | F: GGAGGAAGAAGGTCTTCGG | 248 | Fitzgerald (2018) |
| R: AATTCCGCCTACCTCTGCACT | |||
| Bacteroides | F: GGTGTCGGCTTAAGTGCCAT | 140 | Gregory (2015) |
| R: CGGAYGTAAGGGCCGTGC |