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Characteristics of the primers used for real-time PCR analysis.
| Genes | Primer (from 5′ to 3′) | Product size (bp) | Accession no. |
| Claudin-1 | F:CTCAATACAGGAGGGAAGCCA | 91 | NM-001244539.1 |
| R: ATATTTAAGGACCGCCCTCTCC | |||
| Occludin | F: TCAGGTGCACCCTCCAGATT | 167 | NM-001163647.2 |
| R: TGTCGTTGCTGGGTGCATAA | |||
| ZO-1 | F:ACCATGTCTTGAAGCAGCCA | 103 | XM-021098896.1 |
| R: GCCTGTCTCTCCATGTACGG | |||
| β-actin | F:CTGCGGCATCCACGAAACT | 147 | XM-021086047.1 |
| R: AGGGCCGTGATCTCCTTCTG |
ZO-1, zonula occludens-1.
