Table 2.
Relationship of various concentrations of selected banana as a carbohydrate source and growth curves of each probiotic strain (n = 3)
| Probiotic | Incubation time (h) | Banana powder concentration | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1% | 2% | 3% | ||||||||
| Saba powder | Pisang Awak Banana powder | Silver bluggoe powder | Saba powder | Pisang Awak Banana powder | Silver bluggoe powder | Saba powder | Pisang Awak Banana powder | Silver bluggoe powder | ||
|
Lactobacillus acidophilus CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.79 ± 0.01* | 7.58 ± 0.03* | 7.82 ± 0.01* | 7.79 ± 0.01* | 7.71 ± 0.06* | 7.79 ± 0.01* | 7.49 ± 0.01* | 7.72 ± 0.01* | 7.76 ± 0.03* | |
| 48 | 8.28 ± 0.02* | 7.71 ± 0.00* | 7.92 ± 0.01* | 7.83 ± 0.01* | 7.50 ± 0.00* | 7.81 ± 0.01* | 7.80 ± 0.01* | 7.73 ± 0.03* | 7.88 ± 0.00* | |
| 72 | 8.33 ± 0.01* | 7.77 ± 0.00* | 8.04 ± 0.00* | 8.12 ± 0.01* | 7.88 ± 0.01* | 7.98 ± 0.01* | 8.04 ± 0.01* | 7.56 ± 0.00* | 8.01 ± 0.00* | |
|
Lactobacillus casei CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.83 ± 0.00* | 7.71 ± 0.01* | 7.82 ± 0.00* | 7.74 ± 0.00* | 7.70 ± 0.00* | 7.72 ± 0.01* | 7.70 ± 0.01* | 7.70 ± 0.01* | 7.64 ± 0.02* | |
| 48 | 7.91 ± 0.00* | 7.74 ± 0.00* | 7.91 ± 0.00* | 7.84 ± 0.00* | 7.81 ± 0.02* | 7.74 ± 0.00* | 7.85 ± 0.00* | 7.96 ± 0.00* | 7.76 ± 0.01* | |
| 72 | 7.95 ± 0.01* | 8.19 ± 0.00* | 8.22 ± 0.00* | 7.91 ± 0.01* | 8.11 ± 0.00* | 8.08 ± 0.00* | 8.21 ± 0.00* | 8.11 ± 0.00* | 8.19 ± 0.00* | |
|
Lactobacillus fermentum CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.52 ± 0.01* | 7.60 ± 0.02* | 7.69 ± 0.01* | 7.59 ± 0.01* | 7.70 ± 0.01* | 7.80 ± 0.01* | 7.52 ± 0.01* | 7.74 ± 0.02* | 7.70 ± 0.01* | |
| 48 | 7.84 ± 0.01* | 7.81 ± 0.01* | 7.73 ± 0.01* | 7.66 ± 0.00* | 8.19 ± 0.01* | 7.92 ± 0.01* | 7.90 ± 0.00* | 8.13 ± 0.01* | 7.79 ± 0.01* | |
| 72 | 8.08 ± 0.00* | 8.12 ± 0.00* | 8.19 ± 0.00* | 7.89 ± 0.00* | 8.19 ± 0.00* | 7.95 ± 0.00* | 8.25 ± 0.00* | 8.23 ± 0.00* | 7.80 ± 0.01* | |
|
Streptococcus thermophiles CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.79 ± 0.01* | 7.47 ± 0.03* | 7.46 ± 0.01 | 7.37 ± 0.02* | 7.16 ± 0.05* | 7.47 ± 0.02* | 7.71 ± 0.01* | 7.31 ± 0.02* | 7.69 ± 0.01* | |
| 48 | 7.77 ± 0.01* | 7.58 ± 0.01* | 7.50 ± 0.01 | 7.47 ± 0.01* | 7.33 ± 0.02* | 7.64 ± 0.01* | 7.72 ± 0.02* | 7.67 ± 0.01* | 7.90 ± 0.01* | |
| 72 | 8.01 ± 0.00* | 8.28 ± 0.01* | 7.57 ± 0.01 | 7.77 ± 0.01* | 7.98 ± 0.01* | 7.74 ± 0.01* | 7.76 ± 0.01* | 7.89 ± 0.00* | 8.02 ± 0.00* | |
| Probiotic | Incubation time (h) | Banana powder concentration | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 4% | 5% | 6% | ||||||||
| Saba powder | Pisang Awak Banana powder | Silver bluggoe powder | Saba powder | Pisang Awak Banana powder | Silver bluggoe powder | Saba powder | Pisang Awak Banana powder | Silver bluggoe powder | ||
|
Lactobacillus acidophilus CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.16 ± 0.02* | 7.61 ± 0.00* | 7.57 ± 0.01* | 7.39 ± 0.01* | 7.45 ± 0.04* | 7.84 ± 0.01* | 6.38 ± 0.02* | 7.44 ± 0.00* | 7.54 ± 0.00* | |
| 48 | 8.00 ± 0.01* | 7.67 ± 0.01* | 7.82 ± 0.01* | 7.82 ± 0.01* | 7.58 ± 0.07* | 7.95 ± 0.00* | 7.85 ± 0.00* | 7.78 ± 0.19* | 8.06 ± 0.01* | |
| 72 | 8.21 ± 0.01* | 7.99 ± 0.01* | 8.09 ± 0.00* | 8.24 ± 0.01* | 7.76 ± 0.01* | 8.16 ± 0.00* | 7.90 ± 0.01* | 7.93 ± 0.01* | 8.26 ± 0.00* | |
|
Lactobacillus casei CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.76 ± 0.01* | 7.82 ± 0.00* | 7.65 ± 0.01* | 7.70 ± 0.01* | 7.80 ± 0.00* | 7.59 ± 0.01* | 7.84 ± 0.00* | 7.68 ± 0.00* | 7.65 ± 0.00* | |
| 48 | 7.82 ± 0.00* | 7.89 ± 0.00* | 7.92 ± 0.00* | 7.82 ± 0.01* | 8.01 ± 0.00* | 7.91 ± 0.00* | 7.90 ± 0.00* | 7.75 ± 0.01* | 7.89 ± 0.01* | |
| 72 | 8.13 ± 0.00* | 8.08 ± 0.00* | 7.99 ± 0.00* | 8.07 ± 0.00* | 8.17 ± 0.00* | 8.21 ± 0.00* | 8.07 ± 0.00* | 7.79 ± 0.00* | 8.15 ± 0.00* | |
|
Lactobacillus fermentum CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.70 ± 0.01* | 7.83 ± 0.00* | 7.62 ± 0.01* | 7.70 ± 0.01* | 7.77 ± 0.00* | 7.74 ± 0.01* | 7.53 ± 0.01* | 7.73 ± 0.01* | 7.57 ± 0.01* | |
| 48 | 7.96 ± 0.00* | 8.18 ± 0.01* | 8.04 ± 0.00* | 7.82 ± 0.01* | 7.83 ± 0.01* | 7.76 ± 0.01* | 7.69 ± 0.01* | 7.76 ± 0.01* | 7.82 ± 0.01* | |
| 72 | 8.18 ± 0.00* | 8.19 ± 0.00* | 8.18 ± 0.00* | 8.07 ± 0.00* | 7.86 ± 0.01* | 8.07 ± 0.01* | 7.73 ± 0.00* | 8.06 ± 0.01* | 7.87 ± 0.01* | |
|
Streptococcus thermophiles CFU/mL |
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| 24 | 7.41 ± 0.02* | 7.44 ± 0.01* | 7.42 ± 0.02* | 7.42 ± 0.02* | 7.16 ± 0.00* | 7.42 ± 0.02* | 7.29 ± 0.02* | 7.37 ± 0.01* | 7.73 ± 0.01* | |
| 48 | 7.64 ± 0.01* | 7.58 ± 0.01* | 7.63 ± 0.02* | 7.67 ± 0.01* | 7.77 ± 0.01* | 7.71 ± 0.01* | 7.74 ± 0.01* | 7.71 ± 0.01* | 7.74 ± 0.01* | |
| 72 | 7.67 ± 0.01* | 8.17 ± 0.00* | 7.94 ± 0.00* | 7.75 ± 0.01* | 8.12 ± 0.01* | 8.14 ± 0.00* | 7.97 ± 0.01* | 8.32 ± 0.01* | 7.77 ± 0.01* | |
*p ≤ 0.05 denotes an increase of described parameter. A significant increase of probiotic numbers from baseline (0 h fermentation)