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. 2019 Dec 13;19(1):13–27. doi: 10.1007/s40200-019-00469-0

Fig. 2.

Fig. 2

Bioinformatics analysis of miR29b-HDAC4 and Luciferase assay for confirmation of target prediction. a. The low binding energy between miR-29b and HDAC4 shows high stability of interaction as per the FindTar analysis. b. Binding position prediction of miR29b with HDAC4 by TargetScan. c. Secondary structure prediction of miR29b with HDAC4 by RNAhybrid. Red sequence: HDAC4; Green sequence: miR-29b-3p. d. In comparison to pmirGLO vector without insert, there was a significant increase in luciferase activity of pmirGLO vector with HDAC4 insert transfected with miR-29b mimics. Data are expressed as the mean ± SD calculated from three experiments. *** depicts P < 0.001 in comparison to the control group (n = 3)