Fig. 5.

Effect of miR-29b mimics on expression patterns of miR-29b, HDAC4, TGF-β1, and COL4A1. a. Relative expression levels of miR29b were increased on transfection with miR29b mimics. b. Relative mRNA expression levels of HDAC4 were significantly downregulated in the miR-29b mimics treated group when compared with the control group as per real-time PCR analysis. c & d. Protein expression levels of HDAC4 were decreased in the miR-29b mimics treated group when compared with the control group as per western blotting analysis. e & f. Protein expression levels of TGF-β1 were decreased in the miR29b mimics treated group as per western blotting analysis. g. Protein expression levels of collagen type-IV were decreased in the miR29b mimics treated group when analyzed through ELISA. Data are expressed as the mean ± SD calculated from three experiments. * depicts P < 0.05 and ** depicts P < 0.01 in comparison to the control group (n = 3). HG, High glucose; TGF-β1, Transforming growth factor-β1