Fig. 7.

Effect of miR-29b mimics on macrophage-mediated podocyte impairment. a. Relative miR-29b expression levels were increased in the miR-29b mimics treated as compared to the untreated groups under hyperglycaemic condition as per the real-time PCR analysis. b. Relative mRNA expression levels of HDAC4 were downregulated in the miR-29b mimics treated co-culture of podocytes and macrophages under hyperglycaemic conditions as compared to the untreated groups under hyperglycaemic condition when analyzed through real-time PCR. c. Expression patterns of HDAC4, COL4A1 & TGF-β were increased in the co-culture of podocytes and macrophages under hyperglycaemic conditions as compared to the control group. d. The IL-6 levels were reduced in the miR-29b mimics treated group when analyzed through ELISA. Data are expressed as mean ± SD calculated from three experiments. * depicts P < 0.05, ** depicts P < 0.01 and *** depicts P < 0.001 in comparison to the control group. HG, High glucose; TGF-β1, Transforming growth factor- β1; HG, High glucose; IL-6, Interleukin-6; COL4A1, Collagen type-IV (n = 3)