Fig. 8.

Effect of miR-29b mimics on renal fibrosis and expression patterns of HDAC4, COL4A1 & TGF-β in the in-vivo DN model. a. Decreased expression of miR-29b in diabetic condition was found to be elevated in the miR-29b mimics treated group (n = 5). b. Protein expression levels of HDAC4, COL4A1 and TGF-β1 were downregulated in the miR-29b mimics treated group in comparison to control group when analyzed through western blotting. c. Trichome staining showed that miR-29b mimics treatment decreased renal fibrosis as compared to the control group as per the analysis by ImageJ. d. Hematoxylin and Eosin staining showed that miR-29b mimics treatment decreased glomerular damage as compared to control as per the analysis by ImageJ. In c & d, 1 to 4 is assigned as- 1: Control, 2: Diabetic, 3: Negative control, 4: Diabetic + miR-29b mimics treated group. Data are expressed as the mean ± SD calculated from three experiments. * depicts P < 0.05 and ** depicts P < 0.01 in comparison to the control group (n = 6). TGF-β1, Transforming growth factor- β1; COL4A1, Collagen type-IV