Figure 6.
Screening ROR1-mediated phospho-kinase signaling pathways. Detecting the relative phosphorylation levels of 43 human protein kinases in clone R1 from PC9erlo cell lines by a human phospho-kinase assay kit (A). The relative expression levels of Akt and GSK-3α/β were graphed (B). The phosphorylation levels of Akt, GSK-3α/β, mTOR and β-actin were detected by western blot (C). MN, clone selected from PC9erlo cell lines infected with Lv-shCon; R1, clone 1 selected from PC9erlo cell lines infected with Lv-shROR1.