Figure 4. . Biofilm formation by Scedosporium boydii in the presence of myriocin (4, 8 and 16 μg/ml) or DL-threo-1-Phenyl-2-palmitoylamino-3-morpholino-1-propanol (32, 64 and 128 μg/ml).

Control cells were grown in RPMI 1640 supplemented with 2% methanol. Cells were incubated for 24 h and then the following parameters were measured: biomass by crystal violet staining (A); cell viability by XTT-reduction assay (B); and matrix quantification by safranin staining (C).

*p < 0.05; **p < 0.03; ***p < 0.001.

PPMP: DL-threo-1-Phenyl-2-palmitoylamino-3-morpholino-1-propanol.