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Scedosporium boydii conidia were incubated for 12 h in the presence of myriocin (16 μg/ml) or PPMP (128 μg/ml). Control cells were grown in RPMI 1640 supplemented with 2% methanol. After incubation time, cells were fixed and stained with filipin (50 μg/ml) for 2 h. Samples were analyzed using a fluorescent microscope. (A) Control. (B) Myriocin. (C) PPMP.
PPMP: DL-threo-1-Phenyl-2-palmitoylamino-3-morpholino-1-propanol.
