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. 2020 Mar 30;5(6):891–904. doi: 10.1016/j.ekir.2020.03.020

Figure 6.

Figure 6

Cell viability in HEK293 Tet-on cells overexpressing APOL1 G0, G1, and G2 when treated with Mdivi-1. HEK293 Tet-on G0, G1, or G2 cells were grown without (−) doxycycline (Dox) and with (+) 10 ng/ml Dox for 24 hours. The lactate dehydrogenase assay was used to assess cell viability. Final concentrations of Mdivi-1 (0, 20 μm, and 50 μm) were applied to HEK293 G0, G1, and G2 cells in Dox (−) and Dox (+) conditions (n = 4 in each experiment). Dox induction did not affect cell viability in HEK293 Tet-on G0 cells; G1 and G2 cells had reduced viability after 24-hour Dox induction (P = 0.00008 and 0.00004, respectively). Mdivi-1 did not affect cell viability in HEK293 Tet-on G0, G1, and G2 cells without Dox induction. Mdivi-1 did not alter cell viability in G0 cells with or without Dox-induced APOL1 overexpression. Mdivi-1 (50 μm) restored cell viability in Dox-induced G1 and G2 cells (P = 0.02 and 0.004, respectively); a dose-dependent pattern was seen. Note: Cells with (−)/(+) refer to without or with Dox induction, respectively.