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. 2020 Feb 15;225(4):1193–1224. doi: 10.1007/s00429-020-02041-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 12 — Example of tracing in a genetically engineered mouse ChAT-Cre. Expression of eYFP in cholinergic neurons, switched on through transfection after injection of a modified, double-floxed EF1α–DIO–EYFP–WPRE–pA AAV2 vector (as schematically explained in Fig. 10). a Confocal laser scanning Image at low magnification of an injection spot in the diencephalon–mesencephalon transition (entopeduncular nucleus). b eYFP expressing cholinergic neuron in the boxed area of A scanned at higher magnification. All morphological details of the neuron can be appreciated