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. 2020 Feb 15;225(4):1193–1224. doi: 10.1007/s00429-020-02041-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Anterograde tracing: injection sites and labeling. a, b Case 2012-6 (rat). In the same surgical session, we injected PHA-L in the central caudate putamen (CPu) of the left (L) cerebral hemisphere, while contralaterally (R), we injected BDA in the caudal CPu. Note significant retrograde transport of BDA-to-cerebral cortical pyramidal neurons ipsilateral to the injection site (dashed ellipse). Injection sites measure approximately 1 mm in diameter. c Low-power fluorescence montage in case FS-95166: injection site of PHA-L in the central nucleus (CE) of the amygdaloid complex. Bmg magnocellular basal amygdaloid nucleus. d Low-magnification montage of a combination of PHA-L tracing and neurobiotin injection. PHA-L-labeled fibers (red; Cy3) in layers II–III of medial parahippocampal cortex in contact with apical dendrites of neurobiotin-labeled pyramidal cells (green) located deep (layer V). LD lamina dissecans. e Low magnification: combination of PHA-L tracing and AF555 intracellular injection. An apical dendrite of an intracellularly AF555 injected hippocampal CA1 pyramidal neuron (red) penetrating stratum lacunosum moleculare (LM) The latter contains a terminal field of PHA-L labeled fibers (Alexa Fluor^® 488; green) belonging to the perforant pathway (PHA-L injected in medial parahippocampal cortex). SR stratum radiatum