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. 2020 May 20;5(21):12035–12045. doi: 10.1021/acsomega.0c00244

Figure 4.

Figure 4

HFn–ICG interaction with TfR1high CCs. (a–d) Representative flow cytometry plots showing cell binding with 20, 50, and 100 μg/mL of HFn–ICG (light green, purple, and blue curves, respectively, black curves = control cells) after 2 h incubation at 4 °C. (e–h) Representative confocal microscopy images of cells incubated with HFn–ICG for 2 h at 4 °C to evaluate binding and TfR1 colocalization (blue = cell nuclei stained with DAPI, cyan = cell membrane, green = HFn–ICG, and purple = αTfR1 antibody staining). (i–l) Cellular uptake of HFn–ICG NPs evaluated by IVIS analysis, after incubation at 37 °C for different time points. High TfR1 expression lead to high binding, diffused colocalization with TfR1 (white spots) and strong HFn–ICG uptake.