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Colocalization of HFn–ICG and TfR1. (a,b) Confocal images of MDA-MB 231 cells incubated 15 min, 1, 3, 24, and 48 h at 37 °C in complete cell culture medium with HFn–ICG or free ICG [green; 50 μg/mL; panel (a,b), respectively]. Nuclei were stained with DAPI (blue). TfR1 was recognized with anti-TfR1 antibody (Abcam) and labeled with an anti-rabbit secondary antibody conjugated with Alexa Fluor 488. Scale bar = 50 μm.
