Table 2.
Primers and TaqMan probes used for the experiments
| Gene | Primer sequences (5′–3′) and TaqMan probes | Amplicon size (bp) | Tm (°C) |
|---|---|---|---|
| PAX-7 for | GGTACCGAGAATGATGCGG | 124 | 55 |
| PAX-7 rev | CCCATTGATGAAGACCCCTC | ||
| Pax-7 Probe | 6-FAM/AGCTGATTG /Zen/ACCCGGCCTTGG/3IABkFQ | ||
| MyoD-1 for | GACGTGCCTTCTGAGTCG | 148 | 55 |
| MyoD-1 rev | CTCAGAGCACCTGGTATATCG | ||
| MyoD-1 Probe | 6-FAM/CGCTGCTCT/Zen/CTCCCTCGCTG/3IABkFQ | ||
| Myf-5 for | ATGCCATCCGCTACATCG | 145 | 55 |
| Myf-5 rev | ACAGGACTGTTACATTCGGC | ||
| Myf-5 Probe | 6-FAM/CCCCACCTC/Zen/CAACTGCTCTGAT/3IABkFQ | ||
| MRF-4 for | CCCTGGAATGATCGGAAACA | 95 | 55 |
| MRF-4 rev | CTTCAGCTACAGACCCAAACA | ||
| MRF-4 Probe | 6-FAM/ATCTTGAGG/ZEN/GTGCGGATTTCCTGC/3IABkFQ | ||
| Myogenin for | AGCGAATGCAGCTCTCAC | 150 | 55 |
| Myogenin rev | TGTGATGCTGTCCACGATG | ||
| Myogenin Probe | 6-FAM/TGACCCTAC/Zen/AGATGCCCACAACC/3IABkFQ | ||
| MHC for | GAGTCCTTTGTGAAAGCAACAG | 143 | 55 |
| MHC rev | GCCATGTCCTCGATCTTGTC | ||
| MHC Probe | 6-FAM/CAAGTCTTC/Zen/CCCATGAACCCTCCC/3IABkFQ | ||
| Desmin for | AACGCGATCTCCTCGTTG | 101 | 55 |
| Desmin rev | GAGAACAATTTGGCTGCCTTC | ||
| Desmin Probe | 6-FAM/CAATTCTGC/ZEN/GCTCCAGGTCAATGC/3IABkFQ | ||
| VDR for | CCGCATCACCAAGGACAA | 112 | 62 |
| VDR rev | CTTCCTCTGCACTTCCTCATC | ||
| VDR Probe | 6-FAM/TGTGGACAT/ZEN/CGGCATGATGAAGGA/3IABkFQ | ||
| TRα for | TCCCTAGTTACCTGGACAAAGA | 133 | 59 |
| TRα rev | GGATGGAGGTTCTTCTGGATTG | ||
| TRα Probe | 6-FAM/ACAGCGGTA/ZEN/GTGATAACCAGTTGCC/3IABkFQ | ||
| TRβ for | CTTCCAAACGGAGGAGAAGAA | 115 | 59 |
| TRβ rev | CGTGATACAGCGGTAGTGATAC | ||
| TRβ Probe | 6-FAM/TGTGTAGTG/ZEN/TGTGGTGACAAAGCCA/3IABkFQ | ||
| GCR for | TGGTCCTGTTGTTGCTGTT | 103 | 55 |
| GCR rev | CTTCCCTGGTCGAACAGTTT | ||
| GCR Probe | 6-FAM/TAAGCTCTC/ZEN/CTCCATCCAGCTCCT/3IABkFQ | ||
| IGF-1 for | CAGCAAGTGAGGAGAGGAAC | 131 | 59 |
| IGF-1 rev | GTGTGAGAAGACCACCATCAA | ||
| IGF-1 Probe | 6-FAM/TCGAAGAGA/ZEN/GCAAATGCACATCCCT/3IABkFQ | ||
| LRP-5 for | CCCAGTCTGTCCAGTACATG | 134 | 59 |
| LRP-5 rev | CTCAGAGACCAACCGCATC | ||
| LRP-5 Probe | 6-FAM/CCAACCTCA/ZEN/ATG/3IABkFQ | ||
| LRP-6 for | CCCATTTGTGTTTGATGTCTCC | 137 | 60 |
| LRP-6 rev | CAAGTCTGTCCTTCGAGCTAAA | ||
| LRP-6 Probe | 6-FAM/AAACCTGCA/ZEN/AAGATGGTGCCACAG/3IABkFQ | ||
| PTH-1R for | GGGAAGCCCAGGAAAGATAAG | 125 | 58 |
| PTH-1R rev | CACAGGATGTGGTCCCATT | ||
| PTH-1R Probe | 6-FAM/TGCCTCCTT/ZEN/GTCCTCCTCAGACTC/3IABkFQ | ||
| Irisin for | ACTATGTACTCCGTATCCTCCTC | 126 | 55 |
| Irisin Rev | TGTCATCGGATTTGCCATCT | ||
| Irisin Probe | 6-FAM/CCAGCAGAA/ZEN/GAAGGATGTGTCGGAT/3IABkFQ | ||
| RPS18 for | GATGGCAAAGGCTATTTTCCG | 132 | 60 |
| RPS18 rev | TCTTCCACAGGAGGCCTAC | ||
| RPS18 Probe | 6-FAM/TTCAGGGAT(ZEN/CACTAGAGACATGGCTGC/3IABkFQ |
TaqMan probes with F as reporter fluorochrome (6-carboxyfluorescein [6-FAM]) and ZEN as quencher. Fluorochrome (Iowa Black FQ); bp base pairs of amplicon size; Tm melting temperature (°C)