Table 1.

SYBR® premix Taq™II (2x)	5 μL
PCR forward primer (10 μΜ)	0.1 μL
PCR reverse primer (10 μΜ)	0.1 μL
cDNA	2 μL
RNase-free dH2O	2.8 μL
Total	10 μL

Reaction conditions: 95°C 30 s, 95°C 5 s, 60°C 30s, circle 40 times.