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. 2020 Jun 3;11:2798. doi: 10.1038/s41467-020-16651-5

Fig. 1. Identification of med12 and med13 mutations as morc1 suppressors.

Fig. 1

a Schematic diagram of SDC gene structure and its transcription status in different genetic backgrounds. Open rectangle represents the SDC 5’-untranslated region (5’-UTR). Filled rectangle represents the SDC coding sequence (CDS). b Schematic diagram of the Arabidopsis MED12 and MED13 gene structures with the position of each EMS mutation illustrated. Data display conventions as in a. c GFP fluorescence of plants in different genetic backgrounds. GFP-wt and GFP-morc1 are plants carrying the SDC:GFP transgene in wild-type and morc1 mutant backgrounds, respectively. S243, S213, and S486 are med12 and med13 EMS alleles in the GFP-morc1 background. GFP-morc1/med12 is the med12 CRISPR-CAS9 allele re-created in GFP-morc1 background. GFP-morc1/med13 is the med13 T-DNA allele re-created in GFP-morc1 background. S243-F1 is the F1 resulting from the cross between the CRISPR-CAS9 re-created GFP-morc1/med12 and S243; S213-F1 and S486-F1 are the F1s resulting from the crosses between the re-created GFP-morc1/med13 and S213, S486, respectively. Images show 8-day-old seedlings. The auto-fluorescence (gray) from chloroplasts are shown at the top-right corner of each image. d Real-time PCR quantification of SDC:GFP expression compared between different genetic backgrounds. Each vertical bar represents one biological replicate. A minimum of five biological replicates were analyzed for each mutant background. Red asterisk represents statistically significant difference between the groups under comparison (Student’s t test, two-sided). e GFP fluorescence of plants in different genetic backgrounds. GFP-wt and GFP-ddc are plants carrying the SDC:GFP transgene in wild-type and ddc mutant backgrounds, respectively. GFP-ddc/med12 mutant is the med12 CRISPR-CAS9 allele re-created in GFP-ddc background. GFP-ddc/med13 mutant is the med13 CRISPR-CAS9 allele re-created in GFP-ddc background. f Real-time PCR quantification of SDC:GFP expression compared between different genetic backgrounds. Data display conventions as in d. Source data underlying d, f are provided as a Source data file.