Figure EV4. Molecular model of IFT proteins functions together with HSET for centrosome clustering during mitosis progression.

1. In late G2, dynein helps to maintain the contact between supernumerary centrosomes and the nuclear envelope. Both HSET and dynein, together with IFT proteins, mediate supernumerary centrosome movements around the nucleus.
2. At NEB, dynein/nuclear envelope contacts are lost, HSET together with IFT proteins contribute to maintain inter‐centrosomal cohesion. In the absence of IFT proteins, this activity of HSET is reduced and the average distance between adjacent centrosome increases (gray arrowhead Fig 4D).
3. During mitosis, both dynein and HSET, together with IFT proteins, contribute to focus supernumerary centrosomes into spindle poles. In the absence of IFT proteins, this activity is reduced, at least for HSET, resulting in an increased inter‐centrosomal distance (black arrowhead Fig 4D). Eventually, spindles fail to organize into a bipolar structure, and, in anaphase, the DNA is segregated in more than two DNA mass due to multipolar spindle organization (Figs 1 and EV1 and 4). This abnormal DNA segregation results in reduced cell proliferation in cells naturally harboring supernumerary centrosomes (Fig 5). MT: microtubules. Centro.: centrosomes. Dynein mov.: centrosome movements resulting from dynein activity. HSET mov.: centrosome movements resulting from HSET activity.