Figure 3. Nek2A binds the APC/C^MCC complex when the APC/C^MCC closed conformation is destabilized.

• A
  Ubiquitination reactions of either Nek2A or securin by the APC/C^Cdc20 and the effect of increasing concentrations of the MCC.
• B–F
  Size‐exclusion chromatography peak fractions of APC/C complexes with either Nek2A (B‐E) or securin (F). APC/C^ΔApc15 is the Apc15 deleted mutant APC/C, and MCCΔIR is the Cdc20 IR tail deleted mutant MCC. MCC‐BubR1Wm is the MCC mutant at the APC2^WHB binding surface on BubR1 14. Chromatograms are shown in Fig EV1.

Source data are available online for this figure.