Figure EV3. 3D reconstruction of APC/C–Nek2A complexes.

• A–C
  Workflow for focussed 3D classification and multi‐body refinement for 3D reconstruction and refinement of the cryo‐EM density for the MR tail 1 binding site on Apc8A.
• D–H
  Workflow for focussed 3D classification and multi‐body refinement for 3D reconstruction and refinement of the cryo‐EM density for the repositioned APC2^WHB domain and for the MR tail 2 binding site on Apc2‐Apc4. Classes 2 and 3 shown in (G) differed in the orientation of the APC2^WHB domain, and the occupancy of the MR tail 2 was highest in class 3.