Skip to main content
. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Atherosclerosis. 2011 Aug 6;220(1):45–52. doi: 10.1016/j.atherosclerosis.2011.07.095

Fig. 1.

Fig. 1.

AIF-1 expression is induced by atherogenic stimuli. (A) AIF-1 is not expressed in a normal mouse carotid artery, but is detected in VSMC in lesion from aortic arch of ApoE−/− mouse fed normal chow. Red-brown indicates positive staining. Image is 400×. (B) Fluorescence immunohistochemistry in above lesion. Co-localization of AIF-1antibody (red) with cap and medial VSMC (green), merged, and negative control. Arrows point to examples of co-positive VSMC. 600× magnification for all. (C) Induction ofAIF-1 protein expression in cultured human VSMC by ox-LDL. Human coronary artery VSMC were serum-starved for 48 h, then stimulated with 30 μg ox-LDL for the indicated times. Extracts were blotted with anti-AIF-1 and GAPDH (loading control) antibodies. Western Blot shown is representative.