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Xylitol titer and percent recovery obtained during different steps of downstream processing of fermentation broth from batch cultivation of Y. lipolytica in shake flask on CG/xylose and pure PG/xylose
| Purification steps | CG + Xylose | PG + Xylose | ||
|---|---|---|---|---|
| Xylitol (g/L) | Recovery (%) | Xylitol (g/L) | Recovery (%) | |
| Crude fermentation broth | 48.2 | 100 | 54.0 | 100 |
| Activated charcoal treatment | 37.1 | 76.9 | 39.7 | 73.5 |
| Alcohol precipitation | 24.2 | 49.7 | 27.1 | 52.6 |
| Crystallization | 17.2 | 35.3 | 20.3 | 39.5 |
