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. 2019 Aug 22;105(6):1527–1538. doi: 10.3324/haematol.2019.217414

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Figure 5. — Differences in protein expression between hematopoietic cell subsets. (A) Venn diagram representation of unique and commonly detected proteins using mass spectrometry across healthy T cells, B cells and monocytes. Uniquely detected proteins corresponded to their cellular functions shown in the inset boxes. Out of 1,060 proteins detected in lysates from two healthy and four multiple myeloma (MM) samples, abundance of 753 commonly detected proteins was compared between the three cell types. A complete list of proteins and their corresponding abundance values are provided in Online Supplementary Table S7. (B) Significantly higher expression of S100A8/A9, CAT, IDH1, CES1 and GPX1 was detected in healthy monocytes. (C and D) Heatmap summarizing the expression of proteins that significantly discriminated three cell types in healthy control (HC) and MM samples, respectively (false discovery rate <0.05). The data presented here are normalized label-free quantification (LFQ) intensity values for the proteins.