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. 2019 Oct 3;105(6):1667–1676. doi: 10.3324/haematol.2019.225516

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Figure 1. — Cotl1 is not essential for platelet formation and function under static conditions in vitro. (A and B) Platelet count (A) and size (B) were determined with an automated cell analyzer (ScilVet). (C) Visualization of platelet size and structure using transmission electron microscopy (n=4). Scale bar, 2 μm. (D–F) Platelets were left untreated, lysed, and processed for immunoblotting. Total twinfilin (D), phosphorylated n-cofilin and total n-cofilin (E) were probed with the respective antibodies and analyzed by densitometry (F). GAPDH served as loading control. Values are mean±standard deviation (SD) (n=3). (G) Images of the platelet cytoskeleton ultrastructure on poly-L-lysine. (Left) WT sample. (Right) Cotl1^−/− sample. 0: intact, 1: partially disrupted, 2: strongly disrupted F-actin structures. Scale bar, 1 μm. At least 158 platelets per genotype were analyzed.