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. 2019 Sep 19;105(6):1593–1603. doi: 10.3324/haematol.2019.220160

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Figure 4. — Association between Ki67 and number of follicular helper T cells in follicular lymphoma. (A) The area of the Ki67 binary layer correlates closely with the number of Ki67^pos cells automatically counted (left) and the area of the Ki67 binary layer is significantly higher in grade IIIa or IIIb disease than in grade I-II disease (right), n=99 images from n=23 samples. (B) The number of Ki67^pos cells correlates closely with the number of PD1^pos ICOS^pos cells (left) and there are significantly more follicular helper T cells (T_FH) (as represented by increased area of PD1/ICOS intersection) in grade IIIa and IIIb disease than in grade I-II disease (right), n=42 images from n=13 samples. (C) The degree of T_FH – Ki67 interaction is weakly associated with the number of Ki67^pos cells (left), and the proportion of Ki67^pos cells in contact with T_FH does not differ significantly according to histological grade disease (right) (n=42 images from n=13 samples).