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. 2020 Apr 21;21(6):e50162. doi: 10.15252/embr.202050162

Figure EV2. Loss of tumor‐intrinsic type I IFN is inducible by bone marrow cells and is reversed through HDACi modulation.

Figure EV2

  1. Assessment of tumor‐intrinsic IFN suppression stability over passage (P; number indicated) in culture by qRT–PCR analysis of mean Irf7 and Irf9 mRNA expression in bone‐derived RM1 Irf‐low (RM1 BD Irf−) cells and a reverted (REV) bone‐derived cell line compared to RM1 parental cells. Values are means ± SEM of three independent experiments.
  2. HDACi impact on RM1 BD Irf− proliferation over time by SRB assay. Mean OD at 550 nm (n = 3).
  3. Flow cytometry characterization of bone marrow lymphocyte (%) populations (n = 3).
  4. qRT–PCR of Irf9 expression in parental RM1 cells 48, 72, and 96 h post‐contact co‐culture with FACS‐isolated naïve CD11b+ Ly6G+ BM cells (n = 3 mice per time point).
  5. qRT–PCR of Irf7 expression in RM1 parental cells ± co‐culture with naïve BM ± 48 h treatment with MS275 (n = 3–6). P‐values represented as * < 0.05, ** < 0.005, and *** < 0.0005 (Student's t‐test). All error bars ± SEM.