Figure 6. HDACi and systemic IFN induction provides long‐term immune protection from bone‐metastatic colonization and outgrowth.

• A, B
  FACS analysis of (A) peripheral blood (PB) T and NK lymphocyte representation and activation status and (B) FoxP3⁺ CD4⁺ and CD4⁺ effector memory T‐cell status at days 4 and 10 post‐IC tumor cell inoculation across treatment settings (n = 4–6 per group).
• C, D
  Bioluminescent imaging (C) of a combination group mouse with tumor clearance from days 7 to 41 (full experimental cohort shown in Appendix Fig S5E) post‐IC inoculation with (D) associated specific CD8⁺ memory T‐cell response. This is represented by FACS analysis of IFN‐γ⁺ CD8⁺ T cells (%) post‐ICS induction of T‐cell (spleen derived from tumor‐bearing mice) activation upon re‐stimulation with RM1 BD Irf⁻ cells and MS275, poly I:C, or combination treatments in vitro, with NAC and RM1 BD Irf7 OE controls (n = 3; Irf7 OE control (C), n = 1). Representative FACS plots shown on right.

Data information: P‐values represented as * < 0.05, ** < 0.005, and *** < 0.0005 (Student's t‐test). All error bars ± SEM.