Figure 9. KUP9 regulates intracellular K⁺ and auxin homoeostasis in the root tip.

• A
  ⁺ levels of various materials using the fluorescent dye Asante Potassium Green‐2 (AM). Scale bar, 50 μm.
• B
  Quantification of K⁺ concentration under LK and HK conditions. Data are means ± SE (n = 25, individual plants). Student's t‐test (**P < 0.01) was used to analyze statistical significance, and “#” represents the control.
• C–E
  The contents of free IAA (C), IA‐Asp (D), and IA‐Glu (E) in the root tips of wild type and the kup9‐1 mutant. Data are means ± SE (n = 3, biological replicates; each replicate contains 1,000–1,200 individual plants). Student's t‐test (*P < 0.05, **P < 0.01) was used to analyze statistical significance, and “#” represents the control.
• F
  Phenotype of the kup9‐1/ProKUP9:PIN8 transgenic lines. Seeds were germinated and grown on LK or HK medium for 7 days. Scale bar, 1 cm.
• G
  Primary root length of the plants tested in (F). Data are means ± SE (n = 25, individual plants). Student's t‐test (**P < 0.01) was used to analyze statistical significance.
• H
  QC division rates of the indicated plants. The statistical method is described in Materials and Methods. Data are means ± SE (n = 50–60, individual plants). Student's t‐test (**P < 0.01) was used to analyze statistical significance.

Source data are available online for this figure.