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. 2020 Apr 14;21(6):e49273. doi: 10.15252/embr.201949273

Figure 1. PDS5A distribution in mouse spermatocytes.

Figure 1

  • A–N
    Double immunolabeling of PDS5A (green) and SYCP3 (red) in mouse spread spermatocytes in leptotene (A, B), zygotene (C, D), early pachytene (E, F), mid‐pachytene (G, H), diplotene (I–L), and diakinesis (M, N). Arrows in (I–L) indicate the position of some chromocenters.
  • O, P
    Double immunolabeling of PDS5A (green) and kinetochores revealed with an ACA serum (red) in a metaphase I spermatocyte.
  • Q, R
    Double immunolabeling of PDS5A (green) and REC8 (pseudocolored in purple) in a metaphase I spermatocyte.
  • S, T
    Double immunolabeling of PDS5A (green) and kinetochores (ACA, red) in a metaphase II spermatocyte.
Data information: Sex chromosomes (X, Y) and bivalents (XY) are indicated. DAPI staining of the chromatin (blue) is shown for some spermatocytes. Insets included in (O, Q, and S) correspond to a 300% magnification. Scale bar, 10 μm.