-
A–F
Double immunolabeling of PDS5B (green) and SYCP3 (red) in spread spermatocytes at the indicated stages.
-
G–L
Immunolabeling of PDS5B (green) and either REC8 (pseudocolored in blue, G, H) or SYCP3 (red) and TRF1 (pseudocolored in yellow, I, J) or ACA (pseudocolored in purple, K, L) onto a spread pachytene spermatocyte.
-
M–P
Double immunolabeling of PDS5B (green) and SYCP3 (red) on diplotene (M, N) and diakinesis (O, P) spread nuclei.
Data information: Sex chromosomes (X, Y) and bivalents (XY) are indicated. Insets included in (C–P) correspond to a 300% magnification. Scale bar, 10 μm.