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A, B
Representative images of Pds5AB cKO spread spermatocytes at the indicated stages double immunolabeled for SYCP3 (red) and either PDS5A (green, A) or PDS5B (green, B).
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C, D
Box whiskers plot analyses of corrected total nuclear fluorescence of PDS5A (C) and PDS5B (D) in a given wild‐type (WT, green) or Pds5AB cKO (white) spermatocyte at the indicated stages (n = 10 for each stage). Whiskers indicate the minimum to maximum values, first and third quartiles are depicted by the box, and the horizontal lines in the boxes indicate the median value. Statistical significance was assessed using an unpaired two‐tailed t‐test function (*****P < 0.00001; ns, not significant difference).
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E
Schematic representation of cell progression and timing throughout the indicated stages of spermatogenesis in TX‐treated Pds5AB cKO testes. In mouse, the transition from a differentiating spermatogonia (A1) into a preleptotene cell lasts around 8.6, 8.6 days more to become a pachytene spermatocyte, and finally 17.2 days to progress to elongated spermatids. Note that in seminiferous tubules, this process is continuous and different cell populations coexist. Under our experimental conditions, if the targeted exons were excised in spermatogonial cells, depletion or extreme reduction of PDS5 protein levels would only be accomplished in spermatocytes up to the early pachytene stage (highlighted in red).
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F
Percentage of fluorescence intensity, relative to their respective maximum values, for PDS5A (yellow) and PDS5B (blue) in wild‐type (top) and Pds5AB cKO (bottom) spermatocytes at the indicated stages (n = 10 for each stage).
