Figure EV3. Progression of spermatogenesis in Pds5AB cKO mice.

• A–G
  Immunolabeling of SYCP3 (red) in Pds5AB cKO spread (A–D) and squashed (E–G) spermatocytes at the indicated stages. DAPI staining of chromatin (blue) is shown in (E–G).
• H, I
  DAPI‐stained early (H) and late spermatid (I) nuclei from squashed Pds5AB cKO tubules.
• J–L
  Histological sections of testes from Pds5AB cKO mice.
• M
  Pie graph showing the distribution of prophase I stages after analyzing 1,000 spermatocytes from three individuals of each genotype double immunolabeled for SYCP3 and SYCP1.
• N–P
  Histological sections of testes from wild‐type mice.
• Q
  Pie graph showing the distribution of prophase I stages after analyzing 1,000 spermatocytes from three individuals of each genotype double immunolabeled for SYCP3 and SYCP1.

Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm.