Figure 4. Reduced AEs/LEs length in Pds5AB cKO spermatocytes.

• A–J
  Double immunolabeling of either PDS5A (green, A–E) or PDS5B (green, F–J) and SYCP3 (red) in Pds5AB cKO spread spermatocytes at the indicated stages.
• K–M
  Preparations from the same mice were immunolabeled with the testis‐specific histone variant H1t (green) and SYCP3 (red).
• N
  Scatter dot‐plot graph of total SC length measured in the autosomal pairs of 33 early pachytene cells from three different individuals of each genotype. Bars indicate mean and standard error. Statistical significance was assessed using an unpaired two‐tailed t‐test function (****P < 0.0001).

Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm.