A–ODouble immunolabeling of SYCP3 (red) and the cohesin subunits SMC3 (green, A–C), REC8 (green, D–F), and SMC1β (green, G–I) and cohesin cofactors Sororin (green, J–L) and WAPL (green, M–O) in Pds5AB cKO spread spermatocytes at the indicated stages. Sex bivalents (XY) are indicated. Scale bar, 10 μm.