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. 2020 Apr 14;21(6):e49273. doi: 10.15252/embr.201949273

Figure 8. Altered distribution of telomeric proteins in Pds5AB cKO spermatocytes.

Figure 8

  • A–H
    Double immunolabeling of SYCP3 (red) and either TRF1 (green, A–D) or RAP1 (green, E–H) in Pds5AB cKO spread spermatocytes at the indicated stages.
  • I
    Selected zygotene‐like and pachytene‐like bivalents presenting altered TRF1 distribution as (from left to right) (i) telomere stretches, (ii) multiple telomere, (iii) distant telomeres, and (iv) telomere‐less. Arrowheads in (I) indicate the position of altered telomeres.
  • J
    Quantification of telomeres with a regular or altered disposition of TRF1 in Pds5AB cKO spermatocytes at the indicated stages (n = 1,646 telomeres in zygotene‐like spermatocytes and 738 in pachytene‐like ones).
  • K, L
    As in (I, J), respectively, but for RAP1 staining (n = 1,376 telomeres in zygotene‐like spermatocytes and 328 in pachytene‐like ones). Arrowheads in (K) indicate the position of altered telomeres.
  • M–P
    Double immunolabeling of SUN1 (green) and SYCP3 (red) in Pds5AB cKO spread spermatocytes at the indicated stages. 0, 1, and 2 in (N and O) indicate the number of chromosome ends labeled by SUN1 in a given bivalent.
  • Q
    As in (I), but for SUN1 staining. Arrowheads in (Q) indicate the position of altered telomeres.
  • R
    Selected zygotene‐like and early pachytene‐like bivalents from Pds5AB cKO spermatocytes immunolabeled with SYCP3 (red) and SUN1 (green), and their respective percentage of appearance.
  • S
    Quantification of telomeres with a regular or altered disposition of SUN1 (n = 715 telomeres in zygotene‐like spermatocytes and 410 in pachytene‐like ones).
Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm. For further details on quantification of telomere aberrations, see Appendix Table S1.