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. 2020 Apr 14;21(6):e49273. doi: 10.15252/embr.201949273

Figure 10. Altered attachment of telomeres to the NE in Pds5AB cKO spermatocytes.

Figure 10

  • A–P
    Double immunolabeling of TRF1 (green) and SYCP3 (red) in squashed spermatocytes from wild‐type (A–D, I–L) or Pds5AB cKO mice (E–H, M–P). The first three columns correspond to z‐projections of 15 focal planes throughout the top, equator, and bottom regions of the nucleus. The projection of 65 focal planes across the same spermatocyte nucleus is shown at the fourth column (Z‐projection). White arrowheads in (F and N) indicate the presence of TRF1 signals non‐attached to the NE. Scale bar, 10 μm.
  • Q–T
    Selected bivalents from Pds5AB cKO spermatocytes displaying structural telomere abnormalities as telomere stretches (Q), multiple telomere (R), distant telomere (S), and telomere‐less (T).