FIGURE 5.

Tracheal-rescued ADCK1 mutants are developmentally delayed and have reduced body size. A, Developmental delay was calculated for w¹¹¹⁸ animals, w¹¹¹⁸; ADCK1¹/+ hemizygotes (control), ubiquitously-rescued mutants (ADCK^1/2 da > UAS-ADCK1), tracheal-rescued mutants (ADCK^1/2 btl > UAS-ADCK1), and control animals with tracheal-specific ADCK1 overexpression (w¹¹¹⁸, btl > UAS-ADCK1). The time required for 50% of the population to eclose is shown on the y-axis. Each data point represents results from one collection of animals (each collection n ≥ 150) and the bars depict the mean ± SEM. P values were calculated using one-way ANOVA with Tukey’s multiple comparison test. B, Tracheal-rescued mutant pupae are smaller than controls. Pupal areas were measured and are presented as normalized to the size of w¹¹¹⁸ control pupae. C,D, Protein levels were calculated using a Bradford assay in tracheal-rescued males (C) and females (D). Bars represent mean ± SEM, n = 8 collections of five animals for each genotype. * P ≤ .05, **P ≤ .01, ****P ≤ .0001