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GA exerts protective effects against H/R by decreasing ROS accumulation. (A) Intracellular and (B) mitochondrial accumulation was assessed. (C) Cell viability was assessed using the Cell Counting Kit-8 assay. (D) Cell apoptosis was assessed by Annexin V-FITC/PI double staining. *P<0.05. #P<0.05. GA, glycyrrhizic acid; H/R, hypoxia/reoxygenation; ROS, reactive oxygen species; PI, propidium iodide; NAC, N-acetylcysteine.
