KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-GFP | Thermo Fisher | Cat# A-11122; RRID:AB_221569 |
| Chick anti-Beta galactosidase | Abcam | Cat# ab9361; RRID:AB_307210 |
| Goat anti-Sox2 | Santa Cruz Biotech | Cat# sc-17320; RRID:AB_2286684 |
| Rabbit anti-Sox9 | Millipore | Cat# AB5535; RRID:AB_2239761 |
| Goat anti-OMP | Wako Chemicals | Cat# 544-10001; RRID:AB_664696 |
| Goat anti-Pde2a | Santa Cruz Biotech | Cat# sc-17227; RRID:AB_653928 |
| Donkey anti-Rabbit IgG (H+L), Alexa Fluor 488 | Thermo Fisher | Cat# A-21206; RRID:AB_2535792 |
| Donkey anti-Goat IgG (H+L); Alexa Fluor 555 | Thermo Fisher | Cat# A-21432; RRID:AB_2535853 |
| Goat anti-Rabbit IgG (H+L); Alexa Fluor 555 | Thermo Fisher | Cat# A-21428; RRID:AB_2535849 |
| Goat anti-Chicken IgY (H+L); Alexa Fluor 555 | Thermo Fisher | Cat# A-21437; RRID:AB_2535858 |
| Sheep anti-Digoxigenin-AP, Fab fragments | Sigma-Aldrich | Cat# 11093274910; RRID:AB_514497 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dox Diet, Grain-Based Doxycycline, 200 mg/kg | Bio-Serv | Cat# S3888 |
| Critical Commercial Assays | ||
| VectaShield antifade mounting medium with DAPI | Vector Labs | Cat# H-1200 |
| Click-iT Plus EdU Alexa Fluor 594 picolyl azide toolkit | Thermo Fisher | Cat# C10639 |
| Click-iT Plus EdU Alexa Fluor 647 picolyl azide toolkit | Thermo Fisher | Cat# C10640 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Fgf20GFP-Cre: B6.129-Fgf20tm2.1(cre/EGFP)Dor/J | Huh et al., 2015 | MGI:5751785 |
| Mouse: Fgf20βgal: B6.129-Fgf20tm1.1Dor/J | Huh et al., 2012 | RRID:MGI:5425887 |
| Mouse: ROSAmTmG: B6.129-Gt(ROSA)26Sortm4(ACTB-tdTomato,−EGFP)Luo/J | Muzumdar et al., 2007 | RRID:IMSR_JAX:007576 |
| Mouse: ROSAtdTomato: B6.129-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Madisen et al., 2010 | RRID:IMSR_JAX:007905 |
| Mouse: ROSArtTA: B6.129-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J | Belteki et al., 2005 | RRID:IMSR_JAX:005670 |
| Mouse: TRE-Fgf9-IRES-eGfp: B6.129-Tg(tetO-Fgf9,-EGFP)#Dor/J | White et al., 2006 | MGI:5538516 |
| Mouse: Tcf/Lef:H2b-Gfp: B6.129-Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J | Ferrer-Vaquer et al., 2010 | RRID:IMSR_JAX:013752 |
| Mouse: βCatfl(ex2-6): B6.129-Ctnnb1tm2Kem/J | Brault et al., 2001 | RRID:IMSR_JAX:004152 |
| Mouse: βCatDM: B6.129-Ctnnb1tm3Kba/J | Valenta et al., 2011 | MGI:5308947 |
| Mouse: βCatfl(ex3): B6.129-Ctnnb1tm1Mmt/J | Harada et al., 1999 | MGI:1858008 |
| Recombinant DNA | ||
| Plasmid: pBSKS-Dusp6 | Gift of Dr. Suzanne Mansour (Li et al., 2007) | N/A |
| Software and Algorithms | ||
| ZEN | Zeiss | https://www.zeiss.com/microscopy/us/downloads/zen.html |
| NanoZoomer Digital Pathology (NDP.view2) | Hamamatsu | https://www.hamamatsu.com/jp/en/U12388-01.html |
| Canvas X | ACD Systems | http://www.canvasgfx.com/ |
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |
| Cell Counter plugin for ImageJ | Kurt De Vos | https://imagej.nih.gov/ij/plugins/cell-counter.html |
| Python | Guido van Rossum and Python developers | https://www.python.org/ |
| Pandas | Pandas core team | https://pandas.pydata.org/ |
| NumPy | NumPy developers | http://www.numpy.org/ |
| SciPy | SciPy developers | https://www.scipy.org/ |
| Matplotlib | John Hunter and contributors | https://matplotlib.org/ |
| Statsmodels | Josef Perktold, Skipper Seabold, Jonathan Taylor, and statsmodels developers. | https://www.statsmodels.org/stable/index.html |